|
KMID : 0379120100380010021
|
|
Korean Journal of Mycology
2010 Volume.38 No. 1 p.21 ~ p.24
|
|
|
Selection of High Efficient Enzyme for Protoplasts Isolation from Mushrooms
|
|
Kim Jong-Kun
Kim Jin-Hee
Kong Won-Sik
Kang Hee-Wan
|
|
|
Abstract
|
|
|
|
This study was carried out to select cell wall degrading enzymes for maximizing protoplast yield from Basidiomycetes. The protoplasts were released from spore suspension, mycelia cultured on cellophane membrane, and homogenized mycelia of Flammulina velutipes using commercial cell wall degrading enzymes. The highest yield of protoplasts was obtained from the homogenized mycelia treated with the enzyme combination of GlucanexR 200G and cellulase onozuka R-10. The protocol was also available for Pleurotus ostreatus, P. eryngii, and Hypsizygus marmoreus.
|
|
|
KEYWORD
|
|
|
Basidiomycetes, Cell wall degradation enzymes, Protoplast
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|